醫學論文英翻中

Western blot analysis
Protein was extracted using a buffer containing 50mM Tris-HCI (pH7.4), 2mM EDTA, 100μg/ml leupeptin, 20μg/ml aprotinin, and 100mM NaCl. Fifty microgram of protein per lane was separated by 10% or 12% SDS-PAGE. Primary antibodies were incubated with at appropriate dilutions. Antibody-antigen complexes were detected using the ECL system, and results were analyzed using Bio-Rad GS-700 imaging densitometer.