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DNA論文英翻中
2008/05/16 06:45
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DNA論文英翻中

原文:

We have developed a novel method for detecting DNA methylation status of multiple samples were immobilized on the side and treated with bisulfite directly on the chip. In this experiment, DNAs of pUC19 plasmid were restricted by the enzymes, and ligated with a linker bearing 5’ -terminal acrylamide group at the sticky ends.

 
譯文:

我們已開發出用於消除多個樣本的DNA甲基化現象的新方法,可進行側向固定,且直接切片上添加亞硫酸氫鹽(bisulfite)。在本實驗中,pUC19 質粒的DNA 可由酵素加以限制,且在黏合端使用含5’-丙烯酰胺端的官能基的基因嵌合體(LINKER)

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