Employed by an import 445nm blue laser diode and a qualified optic lens designs inside a durable and sealed metal housing tube, it is designed and manufactured into a professional dot measuring tool of a 445nm blue laser diode module, which is an ideal light source for biological fluorescence imaging, cell analysis, in vivo observation, and optogenetics. being made with basic thermal emitting system inside a different dimension housing tube, after easy connects with an external electric power source and proper integration or installation with biological used machine or device, this blue dot laser alignment enables long term lasted, high stability blue light source generation, ultra clear and precise blue dot projection for both of long term and long distance fields conveniently. It also gets featured advantages of high excitation efficiency, good biocompatibility, fine beam pattern, stability and reliability, and easy integration.

Basically 445nm is the optical excitation wavelength for most biological fluorescent labels, thus 445nm blue laser diode module is resulting in high excitation efficiency, strong fluorescence signal, and high signal-to-noise ratio. It applies an import 445nm blue laser diode within 50mW to 100mW and 150mW to 200mW output power, equipped with a metal heat sink cooling system inside a durable anodized aluminum alloy housing tube with different dimension. This blue dot laser alignment gets sufficient space leaving for tube heat dissipation, highly stable blue light and blue dot projection within daily operating time of 10 hours. Further more, blue laser light is also much gentler than ultraviolet lasers, causing minimal damage to live cells/organisms, suitable for long-term dynamic observation.

In order to make highly precise dot projection at various work distances, 445nm blue laser diode module has been made with a qualified glass coated lens and adjustable focus optic lens. It keeps work with fine beam, high collimation, and extremely small focused dot size, which is suitable for high-precision imaging of single cells and subcellular structures. When it projects high transmittance and high photoelectric ability blue light at several miles far away, on consideration of ultra clear and precise dot alignment, blue dot laser alignment allows clear and precise dot measurement within the maximum work distance of 25 meters and multiple ambient lighting occasions as well. It always ensures reproducible experiments and reliable data, meeting the stringent requirements of scientific instruments.

Applications in biological field:

Fluorescence Microscopy. Confocal laser scanning microscope light source, wide-field fluorescence microscopy, live-cell imaging, and fluorescent protein excitation.

Flow Cytometry. Cell sorting, cell counting, immunophenotyping analysis, rapid detection of fluorescently labeled cells, single-cell analysis

Biofluorescence Spectroscopy. DNA/RNA/protein quantification, fluorescent labeling detection, enzyme activity detection, immunofluorescence analysis, biosensors

In vivo imaging and small animal imaging. Follicular tissue fluorescence imaging in mice/model organisms, tumor, inflammation, cell migration tracing.

Optogenetics. Neuron light control, cell signaling pathway regulation, precise excitation of blue light-responsive photosensitive proteins.

Single-Cell Manipulation and Microfluidics. Single-cell capture, micromanipulation, and light source positioning, microfluidic chip fluorescence detection, particle counting